SimPL Growth of 3T3-L1 Fibroblasts

Growth performance of 3T3-L1 fibroblasts in media containing Simplified Platelet Lysate (SimPL) and fetal bovine serum (FBS). 3T3-L1 fibroblasts were grown in DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS or 10% SimPL. Media supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 5.7 mM Mg. 3T3-L1 cells were passaged using standard techniques in each condition 10 times before seeding a 96-well plate for label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode reader. Data represent counts of cells present within imaged well center. Comparable performance was seen throughout all prior passages without the need for adaptation to media containing SimPL (data not shown).