SimPL Growth of MDCK (NBL-2)

Growth performance of Madin-Darby canine kidney (MDCK NBL-2) cells in media containing Simplified Platelet Lysate (SimPL), fetal bovine serum (FBS), or human platelet lysate (hPL). Cells were grown in standard DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS, 2.5% SimPL, or 2.5% hPL. Media supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 2 mM Mg. Prior to growth evaluation, cells were passaged using standard techniques in 10% FBS with standard DMEM/F12 basal media and were seeded into each condition without adaptation into a 96-well plate. Data represent cell counts relative to the initial seeding density average of each condition obtained from label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode reader.